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Digital Cytology

Bone Marrow Evaluation for Dogs & Cats

When standard bloodwork reveals persistent unexplained cytopenias (low cell counts), abnormal cell populations, or suspected bone marrow disease, bone marrow aspiration and cytologic evaluation is the definitive diagnostic step. Bone marrow is where all blood cells are produced, making it the essential organ to evaluate when blood production is disrupted.

At Sage Veterinary Imaging (SVI), bone marrow samples collected by your veterinarian or at our facilities are evaluated using digital cytology by a board-certified pathologist, providing the detailed assessment needed to diagnose complex hematologic conditions.

SVI offers bone marrow cytology services at our centers in Round Rock, Texas; Spring, Texas; and Sandy, Utah.

Conditions Diagnosed Through Bone Marrow Evaluation

Bone marrow cytology evaluates the production, maturation, and release of red blood cells, white blood cells, and platelets. Abnormalities in any of these cell lines can be identified and classified, guiding specific treatment.

Leukemia

Acute lymphoblastic leukemia Chronic lymphocytic leukemia Acute myeloid leukemia Myelodysplasia

Leukemia — cancer of the blood-forming cells — is diagnosed through bone marrow cytology when abnormal blast cells are identified. Acute leukemias (ALL, AML) are characterized by accumulation of immature blast cells, while chronic leukemias show expansion of more mature cell populations. Bone marrow evaluation determines the leukemia type, blast percentage, and effect on normal cell production, all of which guide treatment decisions and prognosis.

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Non-Regenerative Anemia

Aplastic anemia Pure red cell aplasia Anemia of chronic disease Myelofibrosis

When a pet is anemic and the bone marrow is not producing enough new red blood cells (non-regenerative anemia), bone marrow evaluation identifies the cause. Possibilities include immune-mediated destruction of red cell precursors (pure red cell aplasia), bone marrow failure (aplastic anemia), infiltration by cancer cells (myelophthisis), or chronic inflammatory disease suppressing production. Each cause requires different treatment.

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Thrombocytopenia & Platelet Disorders

Immune-mediated thrombocytopenia Megakaryocyte evaluation Platelet production disorders

Persistent low platelet counts (thrombocytopenia) require bone marrow evaluation to determine whether the problem is decreased production or increased destruction/consumption. Bone marrow cytology evaluates megakaryocyte numbers and maturation — abundant megakaryocytes with peripheral thrombocytopenia suggests immune-mediated destruction (ITP), while decreased or absent megakaryocytes indicates a production problem. This distinction guides very different treatment approaches.

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Bone Marrow Neoplasia & Infiltration

Multiple myeloma Histiocytic sarcoma Metastatic carcinoma Mast cell infiltration

Various cancers can infiltrate the bone marrow, disrupting normal blood cell production. Multiple myeloma (plasma cell cancer) produces characteristic bone marrow findings. Histiocytic sarcoma, metastatic carcinoma, and disseminated mast cell disease can all be identified on bone marrow cytology. Detecting marrow infiltration is important for cancer staging and treatment planning, as it typically indicates advanced disease.

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Infectious & Tick-Borne Diseases

Ehrlichiosis Leishmaniasis Histoplasmosis Cytauxzoonosis

Several infectious diseases affect the bone marrow directly or indirectly. Ehrlichia canis can cause chronic bone marrow suppression. Leishmania organisms may be identified within bone marrow macrophages. Fungal organisms (Histoplasma) can sometimes be found in marrow aspirates. In endemic regions, bone marrow evaluation can be an important diagnostic tool for identifying these infections when blood tests are inconclusive.

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Breeds Commonly Affected

Bone marrow conditions affect all breeds, but some predispositions exist. Golden Retrievers have higher rates of lymphoid leukemia. Bernese Mountain Dogs are predisposed to histiocytic sarcoma, which commonly infiltrates bone marrow. Cavalier King Charles Spaniels have breed-specific thrombocytopenia that can mimic marrow disease. German Shepherds are overrepresented for chronic ehrlichiosis with marrow suppression in endemic areas.

How Digital Cytology Enhances Bone Marrow Evaluation

Bone marrow aspirate smears are complex, containing multiple cell types at various stages of maturation. Digital whole-slide scanning allows our pathologist to systematically evaluate the entire sample, performing differential counts, assessing cellularity, evaluating maturation sequences, and identifying abnormal cell populations. The digital format enables precise, reproducible evaluation and easy archival for comparison with follow-up samples.

Our pathologist provides a comprehensive bone marrow report including cellularity assessment, myeloid:erythroid ratio, maturation evaluation for all cell lines, megakaryocyte evaluation, and identification of any abnormal infiltrates — along with an interpretive summary and clinical recommendations.

What to Expect During Your Visit

Bone marrow aspiration is typically performed by your veterinarian or specialist using a bone marrow needle (most commonly from the proximal humerus or iliac crest). The procedure requires brief general anesthesia or heavy sedation. Aspirate smears are prepared, stained, and submitted to SVI for digital cytology evaluation.

Bone marrow cytology results are typically available within 48–72 hours given the complexity of the evaluation. Rush reads can be arranged for critical cases. The comprehensive report includes clinical correlations and treatment recommendations for your veterinarian.

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Why Choose Sage Veterinary Imaging

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Board-certified pathologist interpretation with expertise in hematologic cytology
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Digital whole-slide scanning for systematic, reproducible bone marrow evaluation
48–72 hour comprehensive reports with rush reads for critical cases
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Complete cell line evaluation including differential counts and maturation assessment
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Three convenient locations in Round Rock TX, Spring TX, and Sandy UT

Get Answers for Your Pet

If your pet has unexplained cytopenias, abnormal blood cells, or suspected bone marrow disease, a bone marrow aspirate evaluated with digital cytology provides the definitive diagnostic information your veterinarian needs.

Submit a Bone Marrow Sample

Contact one of our three locations to learn more about bone marrow cytology services and sample submission.

Round Rock
Austin, Texas Area
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Spring
Houston, Texas Area
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Sandy
Salt Lake City, Utah Area
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Bone Marrow Evaluation FAQ

Bone marrow evaluation is recommended when bloodwork shows persistent unexplained low cell counts (anemia, low white blood cells, low platelets), abnormal circulating cells that may be leukemic, suspected multiple myeloma, or when cancer staging requires evaluation for bone marrow infiltration. Your veterinarian or oncologist will recommend marrow evaluation based on the clinical picture.
Bone marrow aspiration is a routine procedure performed under general anesthesia or heavy sedation. It involves inserting a specialized needle into a bone (usually the humerus or pelvis) and aspirating a small amount of marrow. Complications are rare and typically limited to minor bruising at the collection site. The diagnostic information obtained is invaluable for guiding treatment.
Blood tests evaluate the cells currently circulating in the bloodstream, while bone marrow evaluation examines where blood cells are produced. The marrow shows whether cells are being produced normally, whether production is suppressed, and whether abnormal cells (cancer, infection) are present at the source. Many hematologic diseases cannot be diagnosed without examining the marrow directly.
The myeloid to erythroid (M:E) ratio compares the number of white blood cell precursors to red blood cell precursors in the bone marrow. Abnormal ratios indicate overproduction or underproduction of specific cell lines. For example, a very high M:E ratio may indicate myeloid leukemia, while a very low ratio may suggest red blood cell production is being prioritized in response to severe anemia.
Yes. In immune-mediated thrombocytopenia (ITP), bone marrow shows abundant megakaryocytes (platelet-producing cells) despite low circulating platelet counts, confirming that destruction rather than production failure is the cause. In immune-mediated hemolytic anemia (IMHA), the marrow shows increased red cell production (erythroid hyperplasia) as the body attempts to compensate.
Bone marrow cytology is complex and typically requires 48–72 hours for a comprehensive evaluation. This includes cellularity assessment, differential counts, maturation analysis, and interpretation. For urgent cases (suspected acute leukemia), rush reads can be arranged to provide preliminary results sooner.

Ready to Get Answers?

Bone marrow evaluation with digital cytology can diagnose your pet’s blood disorder and guide effective treatment. Contact one of our locations to get started.

Round Rock
Austin, Texas Area
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Spring
Houston, Texas Area
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Sandy
Salt Lake City, Utah Area
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